Application

Anti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting.

Research CategoryCell Structure

Inhibition Analysis: A representative lot inhibited fertilization in an in vitro system. (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Izumo sperm-egg fusion protein 1 (UniProt: Q9D9J7; also known as Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo) is encoded by the Izumo1 gene (Gene ID: 73456) in murine species. OBF is a single-pass type I membrane protein that is detected on sperm surface after the acrosome reaction. It is an essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. It can be present as a monomer, homodimer, or homooligomer. It is synthesized with a signal peptide (aa 1-21), which is cleaved off in the mature form, which has an extracellular domain (aa 22-319), a short transmembrane domain (aa 320-340), and a cytoplasmic domain (aa 341-397). The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Its interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation. A region comprising of 13 amino acids (148-160) is important for its interaction with IZUMO1R. Mab120 is a fusion-inhibiting antibody that inhibits fertilization. It binds to a region designated as putative functional fragment (aa 5-113). (Ref.: Inoue, N et al. (2013). Development. 140(15); 3221-3229).

Immunogen

Mouse spermatozoa.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in PBS without azide.

Quality

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate.

Specificity

Clone Mab120 is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 (IZUMO1) in murine species. It targets an epitope with in the N-terminal putative functional fragment.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~56 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).